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For that i5 index, evaluate coloration balance using the ahead orientation for MiSeq runs and MiniSeq runs with rapid reagents, and also the reverse-complement orientation for all other devices.
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If running in handbook method and employing bcl2fastq or BCL Change, or uploading the sample sheet to BaseSpace like a manual manner run and attaching a sample sheet, enter the i5 sequence while in the forward direction.
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Set C Index Adapters Seek advice from Index 2 (i5) Orientation for more information regarding how to enter i5 bases around the sample sheet in ahead or reverse complement orientation.
Protection starts off with being familiar with how builders accumulate and share your facts. Facts privateness and stability techniques may well range determined by your use, area, and age. The developer offered this info and may update it over time.
NextSeq a thousand/2000 and NovaSeq X/X Moreover i5 indexes will effectively be output while in the forward orientation for afterwards downstream processing. Custom kits also needs to possess the i5 indexes entered in the ahead orientation.
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